medical technologist

Biju Joseph Ph.D., M.T. (ASCPi)****

**** ******** ******* ***

Ellicott City

MD- 21043-3450

(R) Ph 410-***-****

f2918j@r.postjobfree.com

US visa status: permanent resident with valid employment authorization

Career mission: To start an innovative medical technology course/program

EDUCATION/TRAINING

Institution and location Degree/ Fellowship Year(s) Field of study

Kerala University, India B.S. 1994 Medical Technology

Birla Institute of Technology &

Science, Pilani, India M.S. 1998 Medical Technology

Ph.D. 2006 Life sciences

Johns Hopkins, Post-doctoral fellowship 2007 Cancer Biology

Medicine MD,USA 2010

WORK EXPEREINCE

1994 – 1995

Medical technician/ Medical technologist MGDM Hospital, India /Benziger hospital, India

Hematology/ Chemistry/ Immuno-hematology/microbiology sections

Blood collection; Distributed specimens for hematological, chemistry and blood bank investigations; Operated Merck chemistry analyzer and carried out chemistry analysis; verified blood grouping, Rh typing and cross matching results, Coomb’s test, microbiology samples processing

1999 - 2006

Junior scientist, Vision Research Foundation, India

Blood collection, Clinical testing for RPE65 mutations; Chromosomal analysis and mutation testing in RB; Established DNA diagnostics for RB; Organized blood and tumor collection from RB patients; Blood and tumor processing, DNA isolation, Remedial measures taken in poor quality specimens; Revision of technical manuals

2006 - 2011

Johns Hopkins Medicine Post-doctoral Fellowship, Baltimore, MD 21205,USA

Procured blood and bone marrow of refractory AML cases; RNA and protein isolation from blood and bone marrow samples; Cell culture and harvesting of the pellets for microarray analysis; drug treatment of xenograft tumors to assess therapeutically efficient candidate drugs; Sequencing and Real-time PCR analysis

07/02/2007 -11/06/2007

Senior Research Scientist, AlfaGene Biosciences, Inc., Gaithersburg, MD20877

Transfection grade plasmid isolation; Colored protein and DNA marker development

Professional Honors & Awards:

2000 Senior Research Fellowship - Department of Biotechnology, Government of India

2001 Indian Eye Research Group Travel Fellowship

2002 All India Joint Lectureship Award, Government of India

2008 Maryland Stem cell research Foundation Post-Doctoral Fellowship

2009 July onwards - Book reviewer, Science, Books & Films, published bimonthly by American Association for the Advancement of Science, Washington, D.C. 20005

2010 January - First-ever recipient of Most Valuable Professional award (Chosen automatically based on quality and quantity of posts on Scientistsolutions.com)

2011 American Society of Clinical Pathology Certification –MT (ASCPi)

2011 American Society of Clinical Pathology Member

Personal attributes:

Team player, good in building relationships with colleagues and superiors, ability to adapt to sudden changes in work schedule, demonstrated flexibility, resourceful and hardworking

Technical skills

1. Patient recruitment – maintained working relationship with 250 retinoblastoma (RB) families, pre- and post-genetic testing counseling for the RB patients; organized blood collection for patients at the Genetic counseling clinic

2. Specimen collection and processing

1. Processed hematology/ clinical pathology/ microbiology/chemistry specimens

2. Blood and tumor of RB patients collected during surgery and transported to lab

3. Processed blood/tumor tissue/body fluids for DNA/RNA/microRNA/protein isolation

4. Preparing cultured tumor cells for subcutaneous injection in NOD-SCID mice mammary fat pads

3. Quality checking/ controls /Evaluating new assays

1. Tumor and blood - quantity and quality- DNA isolation/ chromosomal study/clinical chemistry/hematology

2. Quality control sera (internal and external) – analysis of chemistry parameters

3. Genomic DNA quality- gel electrophoresis and quantification

4. Assessed quality of large-scale endotoxin free plasmid isolation- yield, 260/280 OD

4. Protocol development

1. Implemented multiplex PCR for saving limited quantity of genomic and tumor DNA from pediatric patients

2. Verified the results for reporting - Karyotype results in retinoblastoma (13q14 deletion)

3. Prepared reagents and controls for Fluorescent in situ hybridization / mutation screening

4. Compared automated karyotyping with eye karyotyping to assess efficiency

5. Implemented chloroform- iso-amyl alcohol wash step to increase the purity of genomic DNA

6. Optimized FISH analysis on Zeiss work station- RB1 deletion

5. Data analysis and significance testing – sequencing, real-time PCR (mutations, novelty, obtaining accession numbers, submission to databanks, inclusion of suitable controls/standards/statistical analysis)

6. Knowledge of use/ testing/ record keeping and maintenance of lab equipments Micropipettes, semi-automated analyser, flame photometer, electrophoresis units, pH meter, analytical balance, PCR, real-time PCR machine, incubators, refrigerators(Laboratory, Blood-bank), freezers, spectrophotometer ,incubators, water baths

Maintained inventory for sequencing and genotyping reagents, user log, troubleshooting, remedial measures taken (ABI 310/3100 sequencer)

7. Prepared laboratory technical manuals- 1) Cytogenetics and Molecular Biology; Yearly updating of the manuals; 2) Protocol for automated sequencing; 3) Fluorescent in situ hybridization for RB1 deletion analysis

8. Cell culture

Embryonic stem cells (Preparation of mouse embryo fibroblasts, Passaging mouse embryo fibroblasts as matrix for embryonic stem cell cultivation, Freezing / thawing mouse embryo fibroblasts/ embryonic stem cells; Preparing feeder-free conditions (media, plate, TeSR) for embryonic stem cell cultivation, PCR testing for Mycoplasma in cultivated MEF feeders

9. Internship(Bachelors and masters degree in Medical technology)

Identification of pathogenic microorganisms and related diagnostic tests, rotating through all areas in the Microbiology Laboratory, including bacteriology, virology, mycology, mycobacteriology, parasitology, and immunology, Clinical chemistry investigations and interpretation related to suspected clinical diagnosis; Hematological investigations and interpretation of results.

10. Specialized training in virology

Christian Medical College, Vellore, India

Observed various aspects of diagnostic virology which included collection and transport of clinical specimens, virus culture methods, electron microscopy, virus concentration and purification methods, virus antigen and antibody detection methods with special emphasis on the laboratory diagnosis of hepatitis B and Human immunodeficiency virus infections.

11. Specialized training in mycobacteriology

Public health laboratory, Trivandrum, India; Tuberculosis research center, Chennai, India

Technical improvements/Expertise

1. Optimized multiplex PCR for 27-exon RB1 gene in 8 reactions with savings of time, reagents and DNA

2. Optimized sequencing protocols for RPE65 and RB1 screening by uniplex and multiplex PCR

3. Added chloroform-Iso amyl alcohol step to improve purity of DNA from blood

4. In-house preparation of 5X sequencing buffer, and hybridization buffer for fluorescent in-situ hybridization with savings of foreign currency

5. Directed the medical technician in optimizing the culture and metaphase preparations for RB1 deletion analysis by fluorescent in situ hybridization

6. Translation research – RB1 diagnostics, refractory adult AML study

7. 1000 sequencing reactions done for the thesis alone by cycle sequencing chemistry

Familiar softwares

Corel draw, Photoshop, Acrobat, clustal, bioinformatic tools- BLAST, protein modeling using Swiss PDB server, Amino acid conservation analysis, PCR and RT-PCR primer design tools; sequencing and gene mapping softwares, linkage analysis tools- LCP.

Referees

Anirban Maitra, MBBS,

Professor of Pathology and Oncology,

The Sol Goldman Pancreatic Cancer Research Center,

Affiliate Faculty, Department of Chemical and Biomolecular Engineering,

Affiliate Faculty, McKusick-Nathans Institute of Genetic Medicine,

CRB-2, Suite 345, Johns Hopkins University School of Medicine,

1550 Orleans Street, Baltimore MD 21231

Phone: 410-***-**** (Direct) / 410-***-****

Email: f2918j@r.postjobfree.com

Dr. Linda M. Resar,

Associate Prof. (Oncology, pediatrics),

Division of hematology, The Johns Hopkins University School of Medicine,

720 Rutland Avenue,

Ross Research Building, Room 1015, Baltimore, MD.

Ph 410-***-****

Email: f2918j@r.postjobfree.com

Dr. Michael M. Xing,

Associate Professor of Medicine and Oncology,

Division of Endocrinology and Metabolism,

The Johns Hopkins University School of Medicine,

813, Hunterian Street, Baltimore, MD 21287, USA.

Ph. Office: 410-***-****; Research Lab: 410-***-****.

Email: f2918j@r.postjobfree.com

Prof G Kumaramanickavel, MD,

Director of Research, Narayana Nethralaya, Health City, Bangalore, India;

Advisor - Research, Academics and Management, Aditya Jyot Eye Hospital, Mumbai, India;

Visiting Associate, Ophthalmic Genetics & Clinical Services Branch, National Eye Institute, NIH, USA; Formerly: Deputy Director of Research, Professor of Genetics & Molecular Biology, Sankara Nethralaya, India. Email: f2918j@r.postjobfree.com

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