Research/Lab Assistant, Assistant Lab Manager

YINAN FLORRIE ZHU

929-***-**** • adhl3w@r.postjobfree.com • 398 Pacific Street, Brooklyn, NY, 11217

EDUCATION

New York University, New York City, NY Expected May. 2021 Master of Science in Biotechnology

University of California–Davis, Davis, CA June. 2019 Bachelor of Science in Biochemistry and Molecular Biology Bachelor of Arts in Psychology

ACADEMIC EXPERIENCE

Assistant Manager, Biomolecular Science Teaching Lab, New York University Jun 2020 – Present

• DNA sequencing

• Small RNA sequencing

• Running experiments that undergraduates will need to complete in molecular biology and genetics labs in the upcoming semester(s)

• Writing reports for the experiments

• Culturing human primary glioblastoma cells (U-87) as well as cells derived from malignant gliomas (U-118 MG)

• Changing cell medium for both glioblastoma cell lines, U-87 and U-118 MG.

• Organizing and re-stocking chemicals and equipment in the lab

• Handling biological wastes and filling out forms for chemical waste pick-up

• Using i-BUY from NYU Albert to place orders and tracking orders

• Updating inventory list

• Completing other tasks following the instructions given by the professor Graduate Assistant, Biomolecular Science Teaching Lab, New York University Jan 2020 – Present

• Preparing experiments (using kits from EDVOTEK, BIORAD, and ThermoFisher) for the undergraduate advanced molecular and cellular biology as well as microbiology labs

• Attending the laboratory classes to get myself familiarized with the class material

• Overseeing students’ workflow

• Helping the professor with recording experiments during the coronavirus pandemic so that students can watch them at home

• Answering students' questions in person or via emails

• Communicating with professors on a regular basis to check if they need anything else to be done in lab

• Culturing mammalian breast cancer cells (HCC1143) to help the professor of the course (Advanced Molecular and Cellular Biology) with a cytoskeleton fluorescent experiment.

• Switching cell culture medium for the breast cancer cell line (HCC1143)

• Making new medium for the breast cancer cell line (HCC1143)

• Grading students’ quizzes and checking their lab notebooks

• (Starting September 2020) Training new graduate assistants in the lab Research Assistant, Trainor Lab in Department of Psychology, UC Davis Sep 2017 – Jun 2019

• Investigating the downstream effects of oxytocin receptor antagonist in the nucleus accumbens on social withdrawal behavior and dopamine signaling pathways

• Assistant surgeon for p. Californicus mice cannulation and doing perfusion

• Trained in immunohistochemistry (IHC), mounting, and brain slicing, and helping with behavior testing

• Searching literatures through online database (i.e. PubMed, Google Scholar) and studying research papers related to biological psychology and neuroscience

• Attending weekly meeting and discussing research papers Lab Assistant, Draper Lab in Department of Biochemistry and Molecular Biology, UC Davis Jun 2015 – Dec 2016

• Responsible for feeding, checking the experimental objects - zebrafish’s health and getting rid of dead fish

• Observing and helping graduate students with experimental work in lab.

• Cleaning and maintaining equipment in the lab

• Reporting issues to the principal investigator if there was anything wrong with equipment in lab Needs Assessment: Services Available to East Asian Breastfeeding Mothers in Sacramento, CA Research Intern, Liquid Gold Concept Dec 2015 –Jun 2016

• Conducting research on general background of breastfeeding in the US and around the world through searching and reading multiple organizations’ website, literatures, and documents in regard to breastfeeding.

• Composing reports of breastfeeding from research, clinical, communities, and entrepreneurial perspectives

• Drafting an academic poster based on my project and findings and delivering professional presentations Research Assistant, Hunter Lab in Department of Biochemistry and Molecular Biology, UC Davis Apr 2015 – Jun 2015

• Studying the process of meiosis

• Trained in performing Polymerase Chain Reactions (PCR) ACADEMIC PROJECTS

Drug Design, Biotechnology and Pharmaceutical Industry Jan 2020 – Jun 2020

• This whole process of new oral drug exploration guided by Dr. Evgeny Vulfson is to help females of reproductive age (21 – 44 years) get pregnant through the ovulation induction by activating kisspeptin 1 receptor (KISS1R) and thus, increasing the secretion of reproductive hormones, including gonadotropin- releasing hormone (GnRH), follicle-stimulating hormone (FSH), and luteinizing hormone (LH).

• The entire process included: researching disease background, identifying a novel target for oral drug, coming up with a lead structure, researching on appropriate methods to conduct direct target assays and cell assays, coming up new chemical entities (NCE), identifying the best NCE through structural activity relationship

(SAR), identifying both on-and off-target toxicities, drafting the best NCE profile, identifying assays to assess properties of the best NCE, identifying the disease, mechanism, and efficacy biomarker for phase I, identifying the efficacy biomarker for phase II, searching for proper animal disease models, estimating the no observed adverse effect level dose (NOAEL), intermediate dose, and maximum tolerated dose (MTD), calculating therapeutic index (TI), determining human equivalent dose (HED) and maximum recommended starting dose

(MRSD), phase I clinical trial, phase II clinical trial, phase III clinical trial, and creating a target product profile (TPP)-like table for the new drug.

Biosensor Design, Biosensors and Biochips Sep 2019 – Dec 2019

• This biosensor design guided by Dr. Evgeny Vulfson is to help beta-thalassemia patients determine their needs to receive blood transfusion. Beta-thalassemia patients usually need regular blood transfusion, which may lead to excess heavy metals, especially iron, in patients’ blood. Iron overload may cause some severe effects on human bodies. This biosensor helps the patients evaluate their needs to receive blood transfusion through measuring the iron-bound transferrin (Tf) in blood samples.

• The essence of this biosensor relies on enzyme-linked immunosorbent assay (ELISA), which determines both the concentration of Fe3+ bound Tf and the percentage of Fe3+ bound Tf. LEADERSHIP ACTIVITIES

Sponsorship Chair, UC Davis Pre-Health Conference Nov 2017 – Oct 2018

• Holding meetings with directors to assign tasks and follow up progress

• Reminding committee members to finish assigned tasks on a regular basis

• Responsible for building rapport and communicating with sponsors Public Health Chair, UC Davis Pre-Health Conference Nov 2015– Oct 2016

• Arranging meetings and taking notes in the general meetings.

• Overseeing the public health committee

• Being responsible for coming up with new and innovative workshop ideas Public Relation, UC Davis Chapter, Undergraduate American Medical Women’s Association Jun 2015 – Dec 2016

• Attending both e-board and general meetings of the UC Davis chapter

• Writing professional emails to invite speakers to present at the chapter

• Advertising Undergraduate American Medical Women's Association (AMWA) on campus

• Creating and updating both the Facebook page and website of Undergraduate AMWA at UC Davis

• Designing flyers of the AMWA using Adobe and Canva

• Tabling on campus to recruit new members every quarter

• Mentoring freshmen in the chapter

• Training public relation interns

SKILLS&INTEREST

Dry lab: Basic data manipulation and visualization using R studio; Statistic tests: t-test, z-test, ANOVA. Wet Lab: PCR, Agarose Gel Electrophoresis, Western Blot, SDS-PAGE, Chromatography Protein Purification, Microbial Culture and Plasmid Transformation, Organic Synthesis and Compound Analysis, Natural Product Isolation and Purification, IR, Quantitative PCR, DNA Sequencing, Whole RNA Isolation from Cell Cultures, Immunohistochemistry (IHC) Sample Creation and Visualization under Confocal Microscope, DNA Sequencing, Small RNA Sequencing

Lab Equipment: Stereotaxic Apparatus, Cryostat, Centrifuge, Vortex, Confocal Microscope, Spectrophotometer

Computer: Microsoft Excel, Microsoft Word, Microsoft PowerPoint. Languages: English, Mandarin, Italian

Interest: Jogging, Learning Languages, Reading, Writing Blogs, Playing the Piano ADD-ON INFORMATION

Senior Intern, Davis Interfaith Rotating Winter Shelter Sep 2017 – Jun 2019

• Checking guests in and answering guests’ questions in regard to the rules at host sites

• Helping with the security check at the intake

• Updating the guest roster and sleeping bag list

• Checking if the guests have completed their assigned chores on time

• Washing dirty sleeping bags

• Staying up overnight and helping guests if they were sick or had disabilities

• Writing intake report and overnight report after each shift

• Training new interns at the host site

Member and Fundraising Chair, Phi Sigma Pi National Co-ed Honor Fraternity Jan 2016 – Present

• Fundraising chair (Sep 2017 ~ Dec 2017)

o Gathering fundraising ideas from members from the UC Davis Chapter o Contacting local shops and restaurants to arrange fundraising events. o Helping the treasure update the chapter budget form o Writing quarterly reports at the end of the quarter

• General Member

o Attending mandatory events in regard to scholarship, leadership, and fellowship o Helping directors and e-board members with organizing events if necessary Volunteer, Oncology department and gynecology department, Sutter Clinic in Sacramento Oct 2015 – Oct 2016, October 2017 - Oct 2018

• Oncology Department (Oct 2015 – Aug 2016)

• Gynecology Department (Oct 2017 – Aug 2018)

o Picking up phone calls

o Making rounds of patients’ rooms

o Using wheelchairs to transfer elder patients

o Escorting and transporting patients and family members o Assisting patients with diets and double checking their diets by calling their dietitians o Stocking necessities, such as gloves and masks, in the drawers in each patient’s room o Delivering samples (i.e. blood samples, urine samples) to the lab in the clinic Volunteer, Emergency Department, Medical Center in UC Davis Jun 2016 – Sep 2016

• Using wheelchairs to transfer patients

• Cleaning patients’ rooms and gurneys

• Delivering food to patients’ family members

• Delivering blood from the blood center to patients

• Assisting nurses with anything they expected me to do Founding member, Narrative Pre-Health Club Jun 2015 – Mar 2016

• Attending monthly meetings

• Submitting personal artwork in regard to the chapter Intern, UC Davis Chapter, American Society for Biochemistry and Molecular Biology Mar 2015 –Jun 2015

• Assisting e-board members with recruiting members and planning events

• Tabling on campus and distributing chapter flyers

• Making class announcements to advertise events of the chapter

• Writing professional emails to invite speakers

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