Maintenance Quality Control
Resume:
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Neha S. Gajera
SUMMARY:
To work in a challenging but professional atmosphere which shall enable me to keep in touch with emerging technologies, and provide scope for widening the spectrum of my knowledge and thus serve the organization with my expertise and hard work.
SUMMARY:
Advanced levels of proficiency in Excel, PowerPoint, Word and Windows version 95/98/XP/Vista/10. CERTIFICATION OF NATIONAL ASSOCIATION OF PHLEBOTOMY TECHNICIANS (20204902) Aspiring Phlebotomist/Clinical Laboratory Assistance Ø Draw blood from capillaries by dermal puncture, such as heel or finger stick methods Ø Draw blood from veins by vacuum tube, syringe, or butterfly venipuncture methods Ø Organize or clean blood-drawing trays, ensuring that all instruments are sterile, and all needles, syringes, or related
Ø items are of first-time use
Ø Ensuring open channels of communication between patients and employees Ø Performed venous and capillary phlebotomy and ensured specimen integrity Ø Document route of specimens from collection to laboratory analysis and diagnosis Ø Match laboratory requisition forms to specimen tubes Ø Analyzed products i.e. Waste water, raw water, drinking water, cooling water, Demineralized Water
SUMMARY OF TECHNICAL EXPERIENCE:
Molecular biology
• Gene cloning
• PCR
• Recombinant strain development for expression of various enzymes in bacteria, yeast, and fungi.
• Transformation of E.coli and yeast
• Gene isolation, vector construction
• Protein expression studies
• Recombinant expression system-bacterial, yeast and fungi
• Worked on pichia pastoris (yeast) for strain improvement in lipase enzyme Fermentation
• Screening and selection of various enzyme producing micro-organisms of commercial importance.
• Strain improvement using Chemical and UV mutagenesis.
• Maintenance of pure cultures.
• Selection and optimization of culture conditions for bulk production of enzymes.
• Study and evaluate the effect of culture conditions on enzyme production. Protein/Enzyme Detection, separation and purification
• Electrophoresis :- SDS PAGE, Native PAGE
• Protein precipitation techniques
• Qualitative detection of enzyme in polyacrylamide gel using Zymogram
• Partial purification of proteins.
• Protein quantitation: Bradford method, Lowry Method, UV spectroscopy add68r@r.postjobfree.com
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Analytical techniques
• Conducting qualitative and quantitative analysis of a number of enzymes. A few are Cellulases, proteases, Pectinases, Xylanases, Glucosidases, Lactase, Nuclease, Phytase, Lipases, etc. according to various guidelines
Enzyme characterization
• Substrate screening and optimum concentration.
• Determination of kinetic parameters Km, Vo, Vmax, Reaction rate determination, etc.
• Effect of Temperature on activity and stability of enzyme.
• Effect of pH on activity and stability of enzyme.
• Effect of activators/inhibitors.
Microbiology Skills
• Water testing.
• Environment monitoring test by settle plate.
• Microbial media preparation, modification, optimization etc.
• Media suitability and Growth promotion test
• Pure culture screening, isolation, handling, maintenance
• Biochemical tests for microorganisms identifications SUMMARY OF WORK EXPERIENCE:
TG LAB Centennial,CO 12/2019 –2/2020
Research Associate
Ø Creating and conducting protocols for enzymatic testing Ø Creating sop
Ø Testing enzyme lipase,amylase,alkaline protease. Ø Follow Standard Operating Procedures (SOPs) to prepare buffers, reagents and Immunoassay kits Ø Coordinate with outside contractors on lab equipment calibration, maintenance and repair Ø Conducting molecular and biochemical assays with purified proteins Ø Responsible for research and/or development in collaboration with others for assigned projects. Ø Performs lab duties and experiments.
Aumgene Biosciences Pvt. Ltd., Gujarat India 06/2014 – 09/2018
Research Associate
Roles and Responsibilities:
Ø Screening and selection of microorganism for industrially important enzymes Ø Application specific performance evaluation of potential producers and develop commercially viable and economic processes for bulk production
Ø Immunoassay development, optimization and validation Ø Perform experiments and assays to support product troubleshooting and QC Ø Antibody purification and conjugation
Ø Incoming material inspection and testing
Ø Labels and products inspection
Ø Maintain and track supplies and raw material inventory add68r@r.postjobfree.com
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Ø Follow Standard Operating Procedures (SOPs) to prepare buffers, reagents and Immunoassay kits Ø Coordinate with outside contractors on lab equipment calibration, maintenance and repair Ø Communicate with team members and external collaborators Ø Perform mammalian cell culture, qPCR, ELISA, and cell-based assays Ø Maintain lab notebook maintenance and perform general lab maintenance Ø Learn new techniques, plan and execute experiments, and provide input on data interpretation and troubleshooting
Ø Support assay qualification and development activities Ø Performing bacterial growth and plating assays to improve genome editing Ø Designing, constructing, and purifying plasmids containing genome editing reagents Ø Conducting molecular and biochemical assays with purified proteins Ø echPerform all work in compliance with company policy and within the guidelines of BioFire Defense Quality System.
Ø Performs diagnostic tests using PCR to check the functionality of several different instruments produced by BioFire.
Ø Responsible for research and/or development in collaboration with others for assigned projects. Ø Performs lab duties and experiments.
Ø Collects and analyze data relating to real-time PCR. Ø Contributes to project process within his/her scientific discipline. Ø Responsible for maintaining instrument history quality control records. Ø Responsible for developing and revising instrument quality control procedures, involving controlled Word and Excel documents.
Ø Ensures that laboratory notebook for assigned projects/experiments are maintained in accordance with the BioFire Defense Employment Handbook.
. Neopath Dignostic Research Centre pvt. Ltd 09/2013 - 09/2018
. Medical lab technologist
. Roles and Responsibilities:
Ø Provided laboratory analysis of patient specimens Ø Performed quality control, calibration, maintenance, and troubleshooting of equipment per procedures Ø Obtained patient blood samples intravenously or arterially as requested by providers Ø Responsible for in-services on new or revised procedures regarding Hematology and Coagulation Ø Worked as a Laboratory Generalist, capable of working the bench in all areas of the Laboratory including Hematology, Coagulation, Serology, Urinalysis, Chemistry, Microbiology, and Blood Bank Education: Bangalore University, India 08/2011 – 07/2013 Master’s Degree in Science Biotechnology
Metas Adventist College,India 06/2007 – 03/2011
Bachelor’s Degree in Science Biotechnology
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