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Sequencing laboratory technician, Microbiologist

Location:
Johannesburg, Gauteng, South Africa
Salary:
20000
Posted:
November 03, 2024

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Resume:

Terry-Lynn Costello

Berea Street

Pretoria, Muckleneuk, 0002

083*******

*********.********@*****.***

Profile

Enthusiastic microbiologist with a Bachelor’s degree (Honours) and a major in human physiology, bringing three years of hands-on experience as a next-generation sequencing lab technician. Diligent and motivated, I excel at managing workloads and have strong administrative skills that support efficient lab operations. Passionate about advancing scientific research through innovative techniques and collaborative teamwork.

Experience

Next-Generation Sequencing Lab Technician

Inqaba Biotechnical Industries, Pretoria

April 2021 – Present

•Administrative duties include monitoring inventory, updating samples registry, and manage and schedule samples for processing.

•DNA/RNA extraction, and cDNA generation

•PCR, gel electrophoresis, qRT-PCR

•Illumina and Pacific Biosciences Single Molecule Real-Time (SMRT) sequencing library preparation for NextSeq, and PacBio’s Sequel lle, Revio and Onso platforms.

•Omni-C proximity ligation library preparation

•Celemics target enrichment library preparation.

•Sequencing library sizing quality control using TapeStation, Fragment Analyzer.

•Qubit fluorometer and Biospectrophotometer analysis of library.

•Implement and troubleshoot laboratory protocols and workflows.

Education

Bachelor of Science (Honours) in Microbiology

Nelson Mandela University, Port Elizabeth

April 2021

Industrial microbiology – Bioreactors, upscaling production.

Production of secondary metabolites through fermentation

Biochemical and morphological identification of bacteria.

Research project investigating antibiotic-resistant bacteria associated with split-thickness skin graft loss

Bachelor of Science (Microbiology, Physiology)

February 2017 – 2020

Sterility testing and analysis (CFU)

Aseptic techniques

Preparation of microbiological media and operating equipment (nanodrop, autoclave, spectrophotometer, pH meter)

Deutsche Schule Hermannsburg

January 2012 – 2017

Core Mathematics, Physics and Biology

AP English and German second additional language

Head prefect and vice sports captain

Key Skills

Attention to Detail

Time Management

Problem-solving

Excellent communication

Adaptability

Organised

Critical thinking

Interests: Microbial Genomics, Human Physiology, Microbiology, Medical Microbiology

References

Available upon request.



Contact this candidate