Research Assistant- RI Cardiovascular Medicine Garg

A Research position is available immediately for an Assistant with experience in rodent models, molecular biology (western blot, immunohistochemistry, ELISA). Dr. Garg in the Center for Cardiovascular Research and his laboratory focuses on understanding the genetic basis of congenital heart disease and the molecular pathways regulating normal and abnormal cardiac development. We have identified familial cases of congenital heart disease and used traditional linkage studies to discover novel genetic etiologies of human congenital cardiac malformations. In addition, we are interested in understanding how these mutations disrupt normal cardiac development and are generating mouse models to gain insight into the molecular basis of congenital heart disease. Collaboration with other Researchers in the laboratory and the Center will be key.

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Responsibilities

PRINCIPAL DUTIES AND RESPONSIBILITIES

Performs research experiments independently or semi-independently in accordance with protocols approved by the principal investigator. Such experiments will involve the use of laboratory animals (colony management, genotyping, etc.) and may involve to use of human subjects. Intermittent evening and/or weekend duties.

Prepares and tests substrate, conducts assays, and prepares tissue cultures for use in research studies, as applicable. Molecular work including Western blot, RT-PCR and immunocytochemistry as needed.

Ensures accuracy of laboratory analytical procedures as required by specific research projects.

Collects and controls experimental data and maintains accurate daily logs of experiments including methods, results, and conclusions.

Interprets the results of data analyses with input from the principal investigator.

Assists the principal investigator in the preparation of scientific publications and grant proposals.

Make recommendations about refinement and/or replacement of laboratory analytical procedures. Implements such refinements or new procedures with input from the principal investigator.

Orders supplies needed to conduct research; maintains inventory of equipment and supplies; and assures that research equipment is in good working order.

Collaborates in the preparation of research budgets and monitors expenditure of funds to assure that studies are conducted within budgetary constraints.

Instructs other laboratory workers and students in standard methods, practices, procedures, and equipment operations.

Maintains a clean working area and performs general laboratory maintenance. Adheres to proper safety techniques and procedures.

Performs miscellaneous related duties.

Qualifications

KNOWLEDGE, SKILLS AND ABILITIES REQUIRED

Bachelor’s Degree in a relevant field of science from an accredited college.

Six months to two years of relevant laboratory experience. Coursework in anatomy, physiology, statistics, relevant research methods and radiation desirable.

MINIMUM PHYSICAL REQUIREMENTS

1. Percent of time spent on:

50% Preparing test substrates, conducting assays, preparing cultures

25% Interpreting results of data analyses with PI 2.

11% Miscellaneous paperwork associated with research 10% Ordering supplies

02% Preparation of budget

02% Instructing other lab personnel

100% Kneeling / reaching

100% Finger dexterity

100% Talking / hearing / seeing

33% Lifting / carrying weight 0-20 pounds for 1-5 minutes

The above list of duties is intended to describe the general nature and level of work performed by individuals assigned to this classification. EOE M/F/Disability/Vet

Full Time